Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation.
The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.
Participants are recommended to have at least a bachelor’s degree in the life sciences, physical sciences or engineering before attending. Interactions between participants and lecturers will be fostered. Students will have ample opportunity to personally explain their research programs and ask questions about the applicability of specific fluorescence techniques to their course lecturers. Topics addressed in this course include:
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Basic Definitions and Principles of Fluorescence |
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Fluorescence Polarization |
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Time-resolved Fluorescence |
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Instrumentation |
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Data Manipulation and Data Analysis |
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Non-Linear Microscopy Including SHG |
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GFP Fluorescence and Photoactivation |
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Confocal and Multiphoton Fluorescence Microscopy |
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FCS, Fluorescence Correlation Spectroscopy |
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FLIM, Fluorescence Lifetime Imaging |
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Single Molecule Imaging |
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Image Processing and Deconvolution Approaches |
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and More! |
