new trends in advanced fluorescence techniques

january 25-29, 2016 | concepción, chile

1. Basic Definitions and Principles of Fluorescence
   • Basic Spectral Properties
   • Perrin-Jablonski Diagram and Stokes' shift
   • Excitation and Emission Spectra
   • Polarization/Anisotropy
   • Fluorescence Lifetime
   • FRET: Fӧrster Resonance Energy Transfer
2. Confocal and 2-Photon Fluorescence Microscopy
   • Instrumentation
   • Light Sources: One-photon and Multi-photon Excitation
   • Advantages and Disadvantages
3. FCS (Fluorescence Correlation Spectroscopy)
   • Basic Principles of FCS
   • Autocorrelation and PCH Analysis
   • FCS in Solutions and in Cells
4. RICS (Raster Image Correlation Spectroscopy)
   • Basic Concepts
   • RICS in Solutions and Cells
5. N&B (Number and Brightness Analysis)
   • Basic Concepts
   • N&B in Solutions and Cells
6. FLIM (Fluorescence Lifetime Microscopy)
   • The Phasor Approach
   • Applications
7. Particle Tracking
8. Spectral Phasor
9. Practical Use of Instrumentation
10. Overview of Fluorescence Probes and Labeling of Biomolecules - in vitro and in cells