principles of fluorescence techniques 2009
september 14 - 17, madrid, spain
content
(subject to change)
- Basic Definitions and Principles of Fluorescence
- Basic Spectral Properties
- Jablonski Diagram and Stokes' shift
- Excitation and Emission Spectra
- Polarization/Anisotropy
- Fluorescence Lifetime
- FRET: Fluorescence Resonance Energy Transfer
- Instrumentation
- Steady-State Fluorometer
- Instrumentation for Time-Resolved Fluorescence
- Light Sources for Time-Resolved Fluorometry (lamps, lasers, laser diodes, LEDs, synchrotron radiation)
- Detectors (PMT, APD, MCP)
- Time-dependent Phenomena (Part I)
- Multi-Exponential Decays
- Time-Domain Lifetime Measurements
- Frequency-Domain Lifetime Measurements
- Quenching, Static, Dynamic, Transients
- Time-dependent Phenomena (Part II)
- Anisotropy Decays
- Energy Transfer-Distance Distributions
- Time-Dependent Spectral Relaxation
- Excited State Reactions
- Data Manipulation and Data Analysis
- Spectral Manipulation
- Least-square Analysis
- Other Approaches: Maximum Entropy
- Global Analysis
- Analytical Applications of Fluorescence
- Advantages of Fluorescence in Chemical Analysis
- Examples of Fluorescence Assays
- Error Sources in Fluorescence Assays
- Methods of Fluorescence Sensing
- Lifetime-Based Sensing
- Lifetime Sensing and Ratiometric Probes
- Fiber Optics Sensors
- Confocal Fluorescence Microscopy
- Instrumentation
- Light Sources: One-photon and Multi-photon Excitation
- Applications in Cells
- Lifetime Imaging
- Fluorescence Correlation Spectroscopy (FCS)
- FCS in Solutions
- FCS in Cells
- Single-Molecule Fluorescence
- Practical Use of Instrumentation