principles of fluorescence techniques 2009

september 14 - 17, madrid, spain

organizers

Course Coordinator:

Local Course Coordinator:

Organizers

The Microscopy and Dynamic Imaging Unit of the CNIC provides a state-of-the-art optical microscopy laboratory with technical and scientific assistance to all its users. The methodologies developed and employed in the unit range from imaging (immuno-labeling, time-lapse, FRET-FLIM, 3D cross sections, etc.) to different types of fluctuation analysis (diffusion of single proteins, monomer-dimer equilibria, stoichiometry of protein and ligand binding, etc...) as well as particle tracking (vesicle trafficking, wound healing, etc...). Resources are maintained for spectroscopy, microscopy, biochemistry, cell-culture and data analysis. The unit began operations recently in October 2008 under the supervision of Dr. Valeria Caiolfa.

The unit pursues its own cell biophysical research program and is additionally involved in the development of new approaches in optical spectro-microscopy. The main interest is on spatial targeting and assembly of receptors to cell membrane domains, molecular associations, translocations and intracellular trafficking as they vary dynamically in a living cell. These studies are supported by international funds and are based on the use of high resolution imaging tools such as single molecule imaging, time-resolved fluorescence imaging and spectro-microscopy on live cells.