In 1920, F. Weigert discovered that the
fluorescence from solutions of dyes was polarized. Specifically, he looked at solutions of
fluorescein, eosin, rhodamine and other dyes and noted the effect of
temperature and viscosity on the observed polarization. Wiegert discovered that polarization
increased with the size of the dye molecule and the viscosity of the solvent,
yet decreased as the temperature increased.
He recognized that all of these considerations meant that fluorescence
polarization increased as the mobility of the emitting species decreased.