The Beer Lambert Law
ü
üThe absorption strength of a molecule can
be determined by absorption measurements using
The Beer-Lambert Law, which is expressed as:
Absorption (Optical Density) = log Io
/ I = e c
l
ü
Io and
I are the intensities entering and leaving
the
sample respectively
e is
the extinction coefficient or molar absorption index
( a molecular property)
e
l is the pathlength of the sample (1 cm)