advanced fluorescence techniques

september 22-26, 2009 | rio de janeiro, brazil

1. Basic Definitions and Principles of Fluorescence
   • Basic Spectral Properties
   • Jablonski Diagram and Stokes' shift
   • Excitation and Emission Spectra
   • Polarization/Anisotropy
   • Fluorescence Lifetime
   • FRET: Fӧrster Resonance Energy Transfer
2. Instrumentation
   • Steady-State Fluorometer
   • Instrumentation for Time-Resolved Fluorescence
   • Light Sources for Time-Resolved Fluorometry (lamps, lasers, laser diodes, LEDs, synchrotron radiation)
   • Detectors (PMT, APD, MCP)
3. Time-dependent Phenomena (Part I)
   • Multi-Exponential Decays
   • Time-Domain Lifetime Measurements
   • Frequency-Domain Lifetime Measurements
   • Quenching, Static, Dynamic, Transients
4. Time-dependent Phenomena (Part II)
   • Anisotropy Decays
   • Energy Transfer-Distance Distributions
   • Time-Dependent Spectral Relaxation
   • Excited State Reactions
5. Data Manipulation and Data Analysis
   • Spectral Manipulation
   • Least-square Analysis
   • Global Analysis
6. Analytical Applications of Fluorescence
   • Advantages of Fluorescence in Chemical Analysis
   • Examples of Fluorescence Assays
   • Error Sources in Fluorescence Assays
   • Methods of Fluorescence Sensing
   • Lifetime-Based Sensing
   • Lifetime Sensing and Ratiometric Probes
7. Confocal Fluorescence Microscopy
   • Instrumentation
   • Light Sources: One-photon and Multi-photon Excitation
   • Applications in Cells
   • Lifetime Imaging
8. Fluorescence Correlation Spectroscopy (FCS)
   • FCS in Solutions
   • FCS in Cells
   • Single-Molecule Fluorescence
9. Practical Use of Instrumentation